Development of a kompetitive allele‐specific PCR marker for selection of the mutated Wx‐D1d allele in wheat breeding

Waxy (Wx) protein is a key enzyme for synthesis of amylose in endosperm. Amylose content in wheat grain influences the quality of end‐use products. Seven alleles have been described at the Wx‐D1 locus, but only two of them (Wx‐D1b, Wx‐D1e) were genotyped with codominant markers. The waxy wheat line K107Wx1 developed by treating ‘Kanto 107’ seeds with ethyl methanesulphonate carries the Wx‐D1d allele. However, no molecular basis supports this nomenclature. In the present study, DNA sequence analysis confirmed that a single nucleotide polymorphism in the sixth exon of Wx‐D1 changed tryptophan at position 301 into a termination codon. Based on this sequence variation, a PCR‐based KASP marker was developed to detect this point mutation using 68 BC₈F₁ plants and 297 BC₈F₂ lines derived from the cross ‘Ningmai 14’*9/K107Wx1. Combined with codominant markers for the Wx‐A1 and Wx‐B1 alleles, waxy and non‐waxy near‐isogenic lines were distinguished. The KASP marker was efficient in identifying the mutant allele and can be used to transfer waxiness to elite lines.     

无籽刺梨染色体数目观察

以无籽刺梨组培苗根尖为材料，探讨其染色体制片技术。结果表明，无籽刺梨根尖在常温下用0.003mol/L8-羟基喹啉溶液预处理6-8h或0.004mol/L8-羟基喹啉溶液预处理4-6h后，在预冷的卡诺固定液（冰醋酸：无水乙醇=3:1中）0℃下处理15min，经95%乙醇:15% Hcl=1:1的解离液处理5min或无水乙醇:36-38% Hcl=1:1处理6min，然后用卡宝品红染色15或20min，其镜检效果较佳；无籽刺梨的染色体数目为2n=2x=14。无籽刺梨染色体制片技术研究及其染色体数目的确定对其遗传改良工作有着重要的意义。     

库车坳陷克深地区现今地应力场及储层裂缝数值模拟

克深三维区目前处于勘探开发的前期,需要明确地应力场及地下储层裂缝的分布规律,指导开发工作,但该区存在地层埋深大、取心困难、成像测井资料缺乏等实际情况,应力场研究及裂缝预测难度大。利用有限元分析方法,以井点现今地应力值为约束进行了克深三维区现今地应力场的数值模拟,同时建立了应力应变与裂缝参数的定量关系模型,利用古今应力场结合法对克深三维区的裂缝参数进行了预测,模拟结果显示构造高部位和断层带是低应力值区及裂缝的有利发育区。裂缝的数值模拟结果与测井解释结果对比表明,预测结果与实钻吻合度较高,裂缝预测效果较好。     

巧用AutoCAD软件的“三维旋转”命令绘制实体课件

巧妙地应用AutoCAD软件的＂三维旋转＂命令,可以方便快捷地绘制带有倾斜结构及局部剖结构的实体课件,并用两个实例说明了具体的应用方法。     

三维可视化平台下建模分析以及数据的交换——以广州大学为例

使用了三维GIS技术对区域建筑进行虚拟,首先采用ArcGIS to SketchUP6Plugin插件将具有空间参考坐标的广州大学建筑楼的地理数据导入SketchUP,在其中建立带有贴图的精细的广州大学建筑三维模型,最后将三维模型导回Arcscene中进行三维显示及分析。     

基于Unity3d的桂林七星公园全景导航系统

信息可视化技术的使用已经在生活中获得广泛的使用,并且已经在用户中获得了广泛的使用,而将信息可视化技术与三维建模技术结合是近几年来一个新的突破点。本文将介绍如何基于桂林七星公园数字化,并且如何移植到相关移动平台上。该系统主要基于Unity3d平台搭建,结合3dsmax建模技术和使用Ecplips作为开发环境开发的Android技术建立一个方便用户快捷、高速的可视化旅游景区的全景导航系统。     

Interaction of NR2D subunit of NMDA receptor with MOCA

AIM: To identify the potential proteins interacting with NR2D subunit of NMDA receptor by yeast two-hybrid screening and to investigate the role of NR2D in excitotoxicity of the retina.METHODS: The Clontech GAL4 yeast two-hybrid system was used to screen the mouse brain cDNA library, and the bait plasmid containing C-terminus of NR2D was constructed. Physical interaction between 2 proteins was verified by co-immunoprecipitation assay. The subcellular localization of 2 proteins in the mouse retina was observed under microscope with immunofluorescence.RESULTS: Modifier of cell adhesion (MOCA) was identified as a new protein interacting with NR2D. MOCA and NR2D were co-expressed in the mouse retina. CONCLUSION: MOCA specifically interacts with NR2D, which provides the experimental basis for identifying the role of glutamate excitotoxicity in the retina neurodegeneration.     

等壳碾压混凝土重力坝层间抗滑稳定可靠度分析

针对以有限元为基础分析碾压混凝土重力坝层间可靠度其功能函数不能显式表达的特点,采用响应面法、JC法、窄界限法原理相结合的可靠度计算方法。由三维非线性有限元对等壳水电站碾压混凝土重力坝各随机变量分位点值的计算结果,采用抗剪断公式构建该电站非溢流坝段层面的功能函数,求得响应面方程,并应用JC法和窄界限法计算层间单元可靠度指标及层间体系可靠度指标,进一步分析材料的变异系数对体系可靠度的影响。结果表明,φ是影响体系可靠度指标的最敏感因子,碾压各层β值一般为4.16～5.70,层间体系可靠度β'为4.12,满足工程要求。该方法计算效率高,实用性强,用于碾压混凝土重力坝层间抗滑稳定可靠度分析是可行的,值得推广。     

基于Hydrus-1D模型的冬小麦根系层水分渗漏分析

利用Hydrus-1D模型模拟了河北省石津灌区冬小麦水分渗漏情况。结果表明,研究区40％的灌溉降雨量和38％的总供水量流出了200cm边界层,通过设置12种方案得到初始饱和度、2次春灌水量是影响灌区渗漏的主要因素,而二水时间间隔和第一水灌水日期影响相对较小。通过设置426种方案,分别得到了不同初始饱和度时,2次春灌灌水...     

基于工业CT的水泵叶轮三维实体重建

为实现水泵叶轮三维实体重建,利用工业CT获取含有叶轮原型内部构造信息的高精度断层切片图像,并编程实现切片图像RAW数据格式到BMP格式的转换.在VC++6.0平台上,应用MFC、OpenGL、ADO等,通过断层图像预处理、二维图像处理及二维几何处理等数字图像处理技术去除图像噪声并显著提高了图像清晰度;编程完成了叶轮断层...